Медицинская литература


Bacterial P450s and steroid transformations

6,00200 руб.

Рубрика: Генетика. Микробиология

Автор(ы): Kamila du Plessis

Язык: Английский

Издательство: LAP Lambert Academic Publishing

ISBN: 9783844330779

Баркод: 9783844330779

Год: 2011

Наличие книги: Отгружается в течение двух недель

Дополнительные характеристики

Страниц: 156

Описание книги

Four bacterial cytochrome P450 enzyme activities are described in terms of their potential biotechnological use for steroid transformations, by engineered ''Rhodococcus'' strains, into commercially valuable compounds. Three selected rhodococcal P450s were characterized with microbiological, genetic, biochemical, spectroscopic and chromatographical methods. Two sterol C26- hydroxylases were demonstrated to be important for 3- hydroxysterol degradation. The role of the novel dextromethorphan N-demethylase in sterol metabolism was discussed. Furthermore, heterologous production of a mutated P450 enzyme, from ''Bacillus megaterium'', was shown in an engineered ''Rhodococcus'' strain. The activity of the mutated P450 was identified as 16?-hydroxylase of 4- androstene-3,17-dione, by ?H and ??C NMR. The detailed characterization of the cytochrome P450 enzymes, described in this book, provides insight into the catabolic repertoire of selected bacterial strains, necessary for...

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