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DVC methods for assessing living bacterial numbers in natural waters

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ОСТАВИТЬ ЗАЯВКУ

Рубрика: Генетика. Микробиология

Автор(ы): Betty Nwabineli

Язык: Английский

Издательство: LAP Lambert Academic Publishing

ISBN: 9783846539903

Баркод: 9783846539903

Год: 2012

Наличие книги: Отгружается в течение двух недель

Дополнительные характеристики

Страниц: 208

Описание книги

Two DC methods AO and DAPI, were optimised and applied to quantify total populations of bacterial cells in natural waters, with expected differing bacterial numbers, nutritional contents, temperature and salinity. Various culture-based methods were also applied to quantify viable bacterial populations. The two DVC methods optimised in this study, successfully quantified "Living" (Viable culturable and VBNC) bacterial populations in natural waters. NADVCs and INTDVCs were consistently lower than total direct counts and also consistently higher than standard heterotrophic plate counts. Results obtained for cultured bacteria using both DVC methods were similar. However, for mixed bacterial populations, the counts were always higher with the INT method than with the NA method. Combining both DVC methods yielded a statistically significant increase in numbers of INT active cells compared with the INT method used alone. Substrate-enhanced INT method when combined with digitised image...

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