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Purification of Pectin Lyase by Trichoderma harzianum

4,98500 руб.

Рубрика: Генетика. Микробиология

Автор(ы): Irum Mukhtar,Sikander Ali and Rukhsana Bajwa

Язык: Английский

Издательство: LAP Lambert Academic Publishing

ISBN: 9783659116247

Баркод: 9783659116247

Год: 2012

Наличие книги: Отгружается в течение двух недель

Дополнительные характеристики

Страниц: 76

Описание книги

Among different pectinases, pectin lyase seems to be the only pectic enzyme capable of breaking down pectin with a high degree of esterification. Trichoderma harziamun is excellent pectinases producer, therefore different isolates were screened by quantitative and qualitative methods. Screening results showed that two isolates showed maximum pectin lyase. Different fermentation conditions like fermentation rate, inoculum size, concentration of peptone, concentration of yeast extract, pH and tween -80 were optimized by different experiments for production of pectin lyase by T. harzianum isolates. Pectin lyase was partially purified by ammonium sulphate precipitation. The fraction, which contained a large amount of pectin lyase was dialyased against 0.2 M phosphate buffer at pH 5.5 for 12 hours and used for SDS-PAGE and characterization of pectin lyase. The partial purified pectin lyase (PL) from T140 and T 325 isolates had a molecular weight of 22 kDa. Purified enzyme form isolates...

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