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Alkaline Protease Production under Solid State Fermentation

4,98500 руб.
ОСТАВИТЬ ЗАЯВКУ

Рубрика: Генетика. Микробиология

Автор(ы): Gizachew Haile Gidamo

Язык: Английский

Издательство: LAP Lambert Academic Publishing

ISBN: 9783843383387

Баркод: 9783843383387

Год: 2012

Наличие книги: Отгружается в течение двух недель

Дополнительные характеристики

Страниц: 56

Описание книги

A total of 240 alkaliphilic bacterias were isolated from samples collected from alkaline soda lakes of Ethiopia were screened for the production of alkaline proteases. Of these, 30%(80)were protease positive. Out of the 80 protease positive isolates, 20 (25%) grew well and produce appreciable level of enzyme activity when grown in solid state culture. One isolate designated as Bacillus sp.C45 was selected based on enzyme property for further study. The protease produced by Bacillus sp. C45 was characterized to determine its potential industrial application. The enzyme was active in the pH range of 6.5-11.5, with optimum activity at pH 8-9; and stable at alkaline pH. The optimum temperature for activity was 40°C and 50°C in absence and presence of 5mM of Ca+2, respectively. The enzyme also displayed appreciable dehairing activity suggesting potential application for dehairing and detergent at moderate temperatures. By using wheat bran as substrate maximum protease production was...

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